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The copper content of GHK-Cu formulations has been a theoretical safety consideration, given that excessive copper can be toxic - however, the amounts used in cosmetic and research applications are extremely small, the copper is tightly chelated by the peptide (reducing free copper availability), and topical absorption of copper from these products appears minimal and well below levels that would cause systemic copper accumulation or toxicity

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Microbiome imbalances, where alteration in gut and biliary microbiota can affect bile acid metabolism and cholesterol balance, contribute to stone formation [8, 9]

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