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FACS analysis confirmed that at passage 10, 54.7% of EpCAM+ HBs co-expressed the proliferative marker Ki67, and immunostaining showed sustained co-expression of AFP, HNF4, and Ki67

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Importantly, a clinical study has demonstrated that combining radiotherapy with higher doses of the TGF-blocking antibody fresolimumab (10 mg/kg) increases overall survival significantly in metastatic breast cancer patients compared to those undergoing radiotherapy and receiving lower doses of fresolimumab (1 mg/kg) [135]

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