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Consolidation Phase (Weeks 9 to 16+) Extended micro-dosing protocols allow tissue changes to consolidate and stabilize
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TGF-1 induced EMT increases the intracellular levels of unstable iron and ROS, and the elevated iron content and oxidative stress in cells undergoing EMT make them more susceptible to ferroptosis
From there, the islets were placed into 2 ml Eppendorf tubes and frozen at 80 C in TRIzol (Invitrogen) for RNA analysis
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