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2.6 Chromatographic analysis of TMA-related compounds TMA and TMAO were measured through LC-MS/MS analysis using a system composed by an UHPLC Ultimate 3000 (Thermo Fisher Scientific) and a Q-Exactive Plus high-resolution mass spectrometer (Thermo Fisher Scientific)

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Current knowledge is largely derived from preclinical studies investigating the biological processes involved, with a lack of clinical trials confirming these effects in human subjects