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These cell lines were cultured in RPMI1640 medium (Gibco cat#11875119) supplemented with 5% Fetal Bovine Serum (BioWest, cat#S1620), 1% Penicillin/Streptomycin (Fischer Scientific, cat#15-140-122) and 1ug/ml Puromycin (Gibco, cat#A1113802)

Production of reactive oxygen species (ROS) was monitored following 5-(and-6)-chloromethyl-2,7-dichlorodihydrofluorescein diacetate acetyl ester and MitoSOX Redderived fluorescence

Another study reported the interplay between ROS and SIRT1 in cell death during EV71 infection, and inhibition of ROS by NAC was shown to reduce EV71 propagation 149

Erythropoietin Administration for Anemia due to chronic kidney disease - subcutaneous OR intravenous, what do we know so far