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35 12% separating gel (8506.5 l) 4% stacking gel (2213.2 l) 3725 l H2O 1408 l H2O 2125 l seperating gel buffer (1.5 M TrisHCl pH 8.8) 550 l stacking gel buffer (0.5 M TrisHCl pH 6.8) 2138 l 40% acrylamide (37.5:1) 220 l 40% acrylamide (37.5:1) 85 l 10% SDS 22 l 10% SDS 42.5 l 10% APS 11 l 10% APS 8.5 l TEMED 2.2 l TEMED To investigate protein expression in different samples, membranes were sampled with different antibodies

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Patients, meanwhile, have little voice in this process