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Marker gene identification To establish the relationship between cell clusters identified via UMAP analysis and infection stages, we selected genes that are specifically highly expressed at 6 h, 12 h, 18 h, 24 h, 30 h, 36 h, and 42 hpi with red blood cells (RBCs) from the transcriptome data of Plasmodium falciparum 3D7 (GEO Series accession number: GSE150484