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Excess cytoplasmic residues are also the source of reactive oxygen species which can cause damage to sperm membrane, proteins and deoxyribonucleic acid (DNA).[32,33,34] Study on ultrastructure also revealed the presence of Sertoli cells with polymorphic mitochondria with irregular cristae, spermatids with excess cytoplasm, abnormal acrosomes in nicotine treated rats.[35] Another report was also found with curved tail, both bent and curved mid-piece in nicotine treated rats.[21] Moreover, this preliminary study with AFM revealed minute abnormal features of sperm head and neck by representing incandescent resolution and topographic view

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