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For the longitudinal clinical study, ASL perfusion MR images were obtained using a pCASL single-shot echo-planar imaging sequence with the following parameters: TE of 11 ms, TR of 4000 ms, FOV of 220 220 mm, voxel size of 2.75 2.75 mm, slice thickness of 6 mm, 18 slices, a labeling duration of 1650 ms, a post labeling delay of 1600 ms, and 40 control-label pairs

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Besides, in PD mice, FGF-21 suppresses neuroinflammation and apoptosis, enhancing neuronal resilience to metabolic stress and ultimately preserving the functional integrity of the dopaminergic system [104]