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D4H*-mCherry cell labelling and imaging INS-1 832/3 SNAP/FLAG-hGLP-1R WT or V229A cells were seeded onto 13-mm coverslips and labelled with SNAP-Surface 488 probe in full media for 20 minutes before stimulation with 100 nM exendin-4 for 2 minutes at 37C

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The proposed mechanisms are either antibody formation due to massive release of antigens following thyrocyte destruction or generation of new epitopes by a changed and more immunogenic conformation of the Tg molecule with high I 2 content